Journal article
Use of pulsed-field gel electrophoresis for detecting differences in Staphylococcus aureus strain populations between dairy herds with different cattle importation practices
Epidemiology and infection, Vol.129(2), pp.387-395
10/2002
Handle:
https://hdl.handle.net/2376/106218
PMCID: PMC2869898
PMID: 12403115
Abstract
The hypothesis tested was that dairy herds which import cattle for replacement or expansion have a higher prevalence of Staphylococcus aureus mastitis and a greater number of new Staphylococcus aureus strains enter their herds than closed herds. Fifteen commercial dairy herds were divided into four groups based on cattle importation practices. Composite foremilk samples were collected at 4-monthly intervals for 1 year from all lactating cattle. Additionally, foremilk samples were collected from cattle at parturition and skin swabs were taken from the udder of primiparous heifers. All samples were cultured for Staphylococcus aureus and isolates were strain-typed using pulsed-field gel electrophoresis. Herds that purchased replacement heifers had a higher prevalence of Staphylococcus aureus mastitis than herds that purchased lactating cattle for expansion (P = 0.02). Herds that purchased replacement heifers had more total strains of Staphylococcus aureus (P = 0.01) and more new strains (P = 0.04) enter the herd than closed herds.
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Details
- Title
- Use of pulsed-field gel electrophoresis for detecting differences in Staphylococcus aureus strain populations between dairy herds with different cattle importation practices
- Creators
- J. R MIDDLETON - Department of Veterinary Medicine and Surgery, University of Missouri, Columbia, MO 65211, USAL. K FOX - Department of Veterinary Clinical Sciences, Washington State University, Pullman, WA 99164, USAJ. M GAY - Department of Veterinary Clinical Sciences, Washington State University, Pullman, WA 99164, USAJ. W TYLER - Department of Veterinary Medicine and Surgery, University of Missouri, Columbia, MO 65211, USAT. E BESSER - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
- Publication Details
- Epidemiology and infection, Vol.129(2), pp.387-395
- Academic Unit
- Veterinary Clinical Sciences, Department of; Veterinary Microbiology and Pathology, Department of
- Publisher
- Cambridge University Press; Cambridge, UK
- Number of pages
- 9
- Identifiers
- 99900546865901842
- Language
- English
- Resource Type
- Journal article