Use of rMPB70 Protein and ESAT-6 Peptide as Antigens for Comparison of the Enzyme-Linked Immunosorbent, Immunochromatographic, and Latex Bead Agglutination Assays for Serodiagnosis of Bovine Tuberculosis

Hye Cheong Koo; Yong Ho Park; Jongsam Ahn; W. Ray Waters; Mitch V Palmer; Mary Jo Hamilton; George Barrington; Abdelaziz A Mosaad; Kun Taek Park; Woo Kyung Jung; In Yeong Hwang; Sang-Nae Cho; Sang Jae Shin; William C Davis

doi:10.1128/JCM.43.9.4498-4506.2005

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Use of rMPB70 Protein and ESAT-6 Peptide as Antigens for Comparison of the Enzyme-Linked Immunosorbent, Immunochromatographic, and Latex Bead Agglutination Assays for Serodiagnosis of Bovine Tuberculosis

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Use of rMPB70 Protein and ESAT-6 Peptide as Antigens for Comparison of the Enzyme-Linked Immunosorbent, Immunochromatographic, and Latex Bead Agglutination Assays for Serodiagnosis of Bovine Tuberculosis

Hye Cheong Koo, Yong Ho Park, Jongsam Ahn, W. Ray Waters, Mitch V Palmer, Mary Jo Hamilton, George Barrington, Abdelaziz A Mosaad, Kun Taek Park, Woo Kyung Jung, …

Journal of clinical microbiology, Vol.43(9), pp.4498-4506

09/2005

DOI: https://doi.org/10.1128/JCM.43.9.4498-4506.2005

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https://hdl.handle.net/2376/117739

PMCID: PMC1234133

PMID: 16145098

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https://doi.org/10.1128/JCM.43.9.4498-4506.2005View

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Abstract

Clinical Veterinary Microbiology

Current assays used to detect Mycobacterium bovis infection lack accuracy, especially for recently infected animals, or are impractical for rapid field diagnostic applications. To overcome these limitations with serological assays, a synthetic peptide derived from early secretory antigenic target 6 (ESAT6-p) and a recombinant major secreted immunogenic protein (rMPB70) of M. bovis were used in an enzyme-linked immunosorbent assay (EIA), an immunochromatographic assay (ICGA), and a latex bead agglutination assay (LBAA). Sera from noninfected, M. bovis -infected, or M. avium subsp. paratuberculosis -infected (by natural and experimental routes) animals were evaluated. Receiver operating characteristic analysis comparing optical density values from the EIA with results of bacterial culture or skin test, the reference test, established suitable cutoff values for assessing sensitivity and specificity. The EIA and LBAA, respectively, had sensitivities of 98.6 and 94.8%, specificities of 98.5 and 92.6%, and kappa values of 0.97 and 0.88 with ESAT6-p. The EIA, ICGA, and LBAA, respectively, had sensitivities of 96.8, 83.0, and 86.7%, specificities of 90.1, 99.4, and 97.8%, and kappa values of 0.87, 0.85, and 0.83 with rMPB70. Examination of serial samples of sera collected from experimentally M. bovis -infected cattle and deer revealed that ESAT6-p-specific responses developed early after infection whereas responses to rMPB70 developed later in the course of disease. The advantage of the LBAA and ICGA as initial tests for multiple species is a rapid reaction obtained in 2 to 3 h by LBAA or 20 min by ICGA without species-specific secondary antibodies under field conditions, thus allowing immediate segregation of suspect animals for further testing before culling.

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Title: Use of rMPB70 Protein and ESAT-6 Peptide as Antigens for Comparison of the Enzyme-Linked Immunosorbent, Immunochromatographic, and Latex Bead Agglutination Assays for Serodiagnosis of Bovine Tuberculosis
Creators: Hye Cheong Koo - Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
Yong Ho Park - Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
Jongsam Ahn - Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
W. Ray Waters - Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
Mitch V Palmer - Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
Mary Jo Hamilton - Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
George Barrington - Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
Abdelaziz A Mosaad - Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
Kun Taek Park - Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
Woo Kyung Jung - Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
In Yeong Hwang - Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
Sang-Nae Cho - Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
Sang Jae Shin - Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
William C Davis - Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University
Publication Details: Journal of clinical microbiology, Vol.43(9), pp.4498-4506
Academic Unit: Veterinary Microbiology and Pathology, Department of
Publisher: American Society for Microbiology
Identifiers: 99900548312701842
Language: English
Resource Type: Journal article