Use of the mannan receptor to selectively target vaccine antigens for processing and antigen presentation through the MHC class I and class II pathways

W C Davis; R L Konzek; K Haas; D M Estes; M J Hamilton; D R Call; V Apostolopoulos; I F C McKenzie

doi:10.1111/j.1749-6632.2002.tb04362.x

Back

Journal article

Use of the mannan receptor to selectively target vaccine antigens for processing and antigen presentation through the MHC class I and class II pathways

W C Davis, R L Konzek, K Haas, D M Estes, M J Hamilton, D R Call, V Apostolopoulos and I F C McKenzie

Annals of the New York Academy of Sciences, Vol.969(1), pp.119-125

10/2002

DOI: https://doi.org/10.1111/j.1749-6632.2002.tb04362.x

Handle:

https://hdl.handle.net/2376/106985

PMID: 12381575

Abstract

Major Histocompatibility Complex

Adjuvants, Immunologic

Humans

Cells, Cultured

Histocompatibility Antigens Class I - immunology

Parasitic Diseases - prevention & control

Receptors, Mitogen - metabolism

RNA, Messenger - metabolism

Reverse Transcriptase Polymerase Chain Reaction

Cattle - immunology

Animals

Histocompatibility Antigens Class II - immunology

Antigen Presentation

Models, Biological

Antibody Formation

Vaccines, Synthetic

Cytokines - genetics

Immunity, Cellular

Macrophages - immunology

Cytokines - immunology

Extensive studies have shown that synthetic and recombinant vaccines developed against hemoparasites have not been as effective as whole parasites or crude membrane fractions in eliciting protective immunity. A possible reason is that synthetic vaccines are not being presented in a form that induces the appropriate immune response. We have developed a bovine model system to evaluate the ability of adjuvant compounds to induce an immune response to peptide antigens dominated by a cytokine profile with a Type 1 (cell-mediated) or Type 2 (humoral) bias. In the initial testing of this system, we found that mRNA expression of certain cytokines (interleukin [IL]-1beta, IL-6, IL-12, IL-15, GM-CSF, iNOS, and tumor necrosis factor [TNF]-alpha) is enhanced when monocyte-derived macrophages are stimulated with peptide antigen conjugated with mannan under oxidizing conditions compared to peptide conjugated with reduced mannan. The data suggest this model will be useful in identifying adjuvant systems that selectively modulate the cytokine profile of antigen presenting cells at the time of antigen presentation and the consequent downstream maturation of naive T cells to effector cells with Type 1 or Type 2 cytokine bias.

Metrics

14 Record Views

See more details

Details

Title: Use of the mannan receptor to selectively target vaccine antigens for processing and antigen presentation through the MHC class I and class II pathways
Creators: W C Davis - Department of Veterinary Microbiology and Pathology, CVM, Washington State University, Pullman, Washington 99164-7040, USA. davisw@mail.vetmed.wsu.edu
R L Konzek
K Haas
D M Estes
M J Hamilton
D R Call
V Apostolopoulos
I F C McKenzie
Publication Details: Annals of the New York Academy of Sciences, Vol.969(1), pp.119-125
Academic Unit: Department of Veterinary Microbiology and Pathology
Publisher: United States
Identifiers: 99900546826401842
Language: English
Resource Type: Journal article

Use of the mannan receptor to selectively target vaccine antigens for processing and antigen presentation through the MHC class I and class II pathways

Related links

Abstract

Metrics

Details