Use of the "tag-switch" method for the detection of protein S-sulfhydration

Chung-Min Park; Igor Macinkovic; Milos R Filipovic; Ming Xian

doi:10.1016/bs.mie.2014.11.033

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Use of the "tag-switch" method for the detection of protein S-sulfhydration

Journal article

Peer reviewed

Use of the "tag-switch" method for the detection of protein S-sulfhydration

Chung-Min Park, Igor Macinkovic, Milos R Filipovic and Ming Xian

Methods in enzymology, Vol.555, pp.39-56

2015

DOI: https://doi.org/10.1016/bs.mie.2014.11.033

Handle:

https://hdl.handle.net/2376/114164

PMID: 25747474

Abstract

Staining and Labeling - methods

Human Umbilical Vein Endothelial Cells - metabolism

Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) - analysis

Humans

Sulfides - chemistry

Acetates - chemistry

Biotin - chemistry

Spectrometry, Mass, Electrospray Ionization

Human Umbilical Vein Endothelial Cells - cytology

Serum Albumin, Bovine - chemistry

Cysteine - metabolism

Sulfides - metabolism

Sulfides - pharmacology

Streptavidin - chemistry

Serum Albumin, Bovine - analysis

Hydrogen Sulfide - pharmacology

Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) - chemistry

Human Umbilical Vein Endothelial Cells - drug effects

Oxidation-Reduction

Signal Transduction

Jurkat Cells

Hydrogen Sulfide - chemistry

Cysteine - chemistry

Benzothiazoles - chemistry

Protein Processing, Post-Translational

Microscopy, Fluorescence

Protein S-sulfhydration (i.e., converting protein cysteines -SH to persulfides -SSH) is a redox-based posttranslational modification. This reaction plays an important role in signaling pathways mediated by hydrogen sulfide or other reactive sulfane sulfur species. Recently, our laboratories developed a "tag-switch" method which can be used to selectively label and detect protein S-sulfhydrated residues. In this chapter, we provide a comprehensive summary of this method, including the design of the method, preparation of the reagents, validation on small-molecule substrates, as well as applications in protein labeling. Experimental protocols for the use of the method are described in details.

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Title: Use of the "tag-switch" method for the detection of protein S-sulfhydration
Creators: Chung-Min Park - Department of Chemistry, Washington State University, Pullman, Washington, USA
Igor Macinkovic - Department of Chemistry and Pharmacy, University of Erlangen-Nuremberg, Erlangen, Germany
Milos R Filipovic - Department of Chemistry and Pharmacy, University of Erlangen-Nuremberg, Erlangen, Germany. Electronic address: milos.filipovic@fau.de
Ming Xian - Department of Chemistry, Washington State University, Pullman, Washington, USA. Electronic address: mxian@wsu.edu
Publication Details: Methods in enzymology, Vol.555, pp.39-56
Academic Unit: Chemistry, Department of
Publisher: United States
Grant note: R01HL116571 / NHLBI NIH HHS
Identifiers: 99900547308301842
Language: English
Resource Type: Journal article

Use of the "tag-switch" method for the detection of protein S-sulfhydration

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