Utility of Metabolomics toward Assessing the Metabolic Basis of Quality Traits in Apple Fruit with an Emphasis on Antioxidants

Daniel Cuthbertson; Preston K Andrews; John P Reganold; Neal M Davies; B. Markus Lange

doi:10.1021/jf3031088

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Utility of Metabolomics toward Assessing the Metabolic Basis of Quality Traits in Apple Fruit with an Emphasis on Antioxidants

Journal article

Open access

Peer reviewed

Utility of Metabolomics toward Assessing the Metabolic Basis of Quality Traits in Apple Fruit with an Emphasis on Antioxidants

Daniel Cuthbertson, Preston K Andrews, John P Reganold, Neal M Davies and B. Markus Lange

Journal of agricultural and food chemistry, Vol.60(35), pp.8552-8560

09/05/2012

DOI: https://doi.org/10.1021/jf3031088

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https://hdl.handle.net/2376/109537

PMCID: PMC3551554

PMID: 22881116

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Abstract

correlation analysis

apple

hierarchical clustering analysis

metabolomics

quality trait

principal component analysis

A gas chromatography–mass spectrometry approach was employed to evaluate the use of metabolite patterns to differentiate fruit from six commercially grown apple cultivars harvested in 2008. Principal component analysis (PCA) of apple fruit peel and flesh data indicated that individual cultivar replicates clustered together and were separated from all other cultivar samples. An independent metabolomics investigation with fruit harvested in 2003 confirmed the separate clustering of fruit from different cultivars. Further evidence for cultivar separation was obtained using a hierarchical clustering analysis. An evaluation of PCA component loadings revealed specific metabolite classes that contributed the most to each principal component, whereas a correlation analysis demonstrated that specific metabolites correlate directly with quality traits such as antioxidant activity, total phenolics, and total anthocyanins, which are important parameters in the selection of breeding germplasm. These data sets lay the foundation for elucidating the metabolic basis of commercially important fruit quality traits.

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Title: Utility of Metabolomics toward Assessing the Metabolic Basis of Quality Traits in Apple Fruit with an Emphasis on Antioxidants
Creators: Daniel Cuthbertson - Institute of Biological Chemistry and M. J. Murdock Metabolomics Laboratory, Washington State University, P.O. Box 646340, Pullman, Washington 99164-6340, United States
Preston K Andrews - Department of Horticulture and Landscape Architecture, Washington State University, P.O. Box 646414, Pullman, Washington 99164-6414, United States
John P Reganold - Department of Crop and Soil Sciences, Washington State University, P.O. Box 646420, Pullman, Washington 99164-6420, United States
Neal M Davies - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, P.O. Box 646510, Pullman, Washington 99164-6534, United States
B. Markus Lange - Institute of Biological Chemistry and M. J. Murdock Metabolomics Laboratory, Washington State University, P.O. Box 646340, Pullman, Washington 99164-6340, United States
Publication Details: Journal of agricultural and food chemistry, Vol.60(35), pp.8552-8560
Academic Unit: Crop and Soil Sciences, Department of; Horticulture, Department of; Biological Chemistry, Institute of
Grant note: T32 GM008336 || GM / National Institute of General Medical Sciences : NIGMS
Identifiers: 99900547394101842
Language: English
Resource Type: Journal article