Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Babesia bigemina Antibodies in Cattle

Will L Goff; Wendell C Johnson; John B Molloy; Wayne K Jorgensen; Susan J Waldron; Julio V Figueroa; Olivier Matthee; D. Scott Adams; Travis C McGuire; Ignacio Pino; Juan Mosqueda; Guy H Palmer; Carlos E Suarez; Donald P Knowles; Terry F McElwain

doi:10.1128/CVI.00150-08

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Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Babesia bigemina Antibodies in Cattle

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Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Babesia bigemina Antibodies in Cattle

Will L Goff, Wendell C Johnson, John B Molloy, Wayne K Jorgensen, Susan J Waldron, Julio V Figueroa, Olivier Matthee, D. Scott Adams, Travis C McGuire, Ignacio Pino, …

Clinical and vaccine immunology, Vol.15(9), pp.1316-1321

09/2008

DOI: https://doi.org/10.1128/CVI.00150-08

Handle:

https://hdl.handle.net/2376/108102

PMCID: PMC2546671

PMID: 18632921

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https://doi.org/10.1128/CVI.00150-08View

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Abstract

ABSTRACT A competitive enzyme-linked immunosorbent assay (cELISA) based on a broadly conserved, species-specific, B-cell epitope within the C terminus of Babesia bigemina rhoptry-associated protein 1a was validated for international use. Receiver operating characteristic analysis revealed 16% inhibition as the threshold for a negative result, with an associated specificity of 98.3% and sensitivity of 94.7%. Increasing the threshold to 21% increased the specificity to 100% but modestly decreased the sensitivity to 87.2%. By using 21% inhibition, the positive predictive values ranged from 90.7% (10% prevalence) to 100% (95% prevalence) and the negative predictive values ranged from 97.0% (10% prevalence) to 48.2% (95% prevalence). The assay was able to detect serum antibody as early as 7 days after intravenous inoculation. The cELISA was distributed to five different laboratories along with a reference set of 100 defined bovine serum samples, including known positive, known negative, and field samples. The pairwise concordance among the five laboratories ranged from 100% to 97%, and all kappa values were above 0.8, indicating a high degree of reliability. Overall, the cELISA appears to have the attributes necessary for international application.

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Title: Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Babesia bigemina Antibodies in Cattle
Creators: Will L Goff
Wendell C Johnson
John B Molloy
Wayne K Jorgensen
Susan J Waldron
Julio V Figueroa
Olivier Matthee
D. Scott Adams
Travis C McGuire
Ignacio Pino
Juan Mosqueda
Guy H Palmer
Carlos E Suarez
Donald P Knowles
Terry F McElwain
Publication Details: Clinical and vaccine immunology, Vol.15(9), pp.1316-1321
Academic Unit: Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
Identifiers: 99900547473301842
Language: English
Resource Type: Journal article