Journal article
Validation of a low-dose ACTH stimulation test in healthy adult horses
Journal of the American Veterinary Medical Association, Vol.239(6), pp.834-841
09/15/2011
Handle:
https://hdl.handle.net/2376/114429
PMID: 21916767
Abstract
To determine the lowest ACTH dose that would induce a maximum increase in serum cortisol concentration in healthy adult horses and identify the time to peak cortisol concentration.
Evaluation study.
8 healthy adult horses.
Saline (0.9% NaCl) solution or 1 of 4 doses (0.02, 0.1, 0.25, and 0.5 μg/kg [0.009, 0.045, 0.114, and 0.227 μg/lb]) of cosyntropin (synthetic ACTH) were administered IV (5 treatments/horse). Serum cortisol concentrations were measured before and 30, 60, 90, 120, 180, and 240 minutes after injection of cosyntropin or saline solution; CBCs were performed before and 30, 60, 120, and 240 minutes after injection.
For all 4 doses, serum cortisol concentration was significantly increased, compared with the baseline value, by 30 minutes after administration of cosyntropin; no significant differences were detected among maximum serum cortisol concentrations obtained in response to administration of doses of 0.1, 0.25, and 0.5 μg/kg. Serum cortisol concentration peaked 30 minutes after administration of cosyntropin at a dose of 0.02 or 0.1 μg/kg, with peak concentrations 1.5 and 1.9 times, respectively, the baseline concentration. Serum cortisol concentration peaked 90 minutes after administration of cosyntropin at a dose of 0.25 or 0.5 μg/kg, with peak concentrations 2.0 and 2.3 times, respectively, the baseline concentration. Cosyntropin administration significantly affected WBC, neutrophil, and eosinophil counts and the neutrophil-to-lymphocyte ratio.
Results suggested that in healthy horses, administration of cosyntropin at a dose of 0.1 μg/kg resulted in maximum adrenal stimulation, with peak cortisol concentration 30 minutes after cosyntropin administration.
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Details
- Title
- Validation of a low-dose ACTH stimulation test in healthy adult horses
- Creators
- Allison J Stewart - Department of Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA. stewaaj@auburn.eduEllen N BehrendJames C WrightLinda G MartinRobert J KemppainenKatherine A BuschR Reid Hanson
- Publication Details
- Journal of the American Veterinary Medical Association, Vol.239(6), pp.834-841
- Academic Unit
- Veterinary Clinical Sciences, Department of
- Publisher
- United States
- Identifiers
- 99900547686901842
- Language
- English
- Resource Type
- Journal article