Journal article
Validation of an Anaplasma marginale cELISA for use in the diagnosis of A. ovis infections in domestic sheep and Anaplasma spp. in wild ungulates
Veterinary microbiology, Vol.130(1), pp.184-190
2008
Handle:
https://hdl.handle.net/2376/112101
PMID: 18272296
Abstract
A commercially available (cELISA) kit for diagnosing
Anaplasma marginale infection in cattle was validated for diagnosing
A ovis infection in sheep using the bovine serum controls as supplied by the manufacturer (BcELISA) and sheep serum controls from pathogen-free sheep (OcELISA). True positives were identified using two previously established assays, a nested PCR (nPCR) test and an indirect immunofluorescent assay (IFA). The BcELISA was also applied to sera from various species of wild ruminants, comparing the results with the IFA. Receiver operating characteristic (ROC) analysis indicated that the predicted threshold inhibition for the BcELISA was 19.2. The sensitivity for the BcELISA was 98.2% and the specificity was 96.3%. The predicted threshold inhibition decreased to 14.3 for the OcELISA; the sensitivity was 96.5% and the specificity was 98.1%. There was ≥90% concordance between IFA and nPCR, as well as between the BcELISA at 19% inhibition cutoff and either IFA or PCR. Concordance between the cELISA and IFA using sera from elk, mule deer, bighorn sheep, pronghorn antelope, and black-tailed deer ranged from 64% to 100%. This commercially available cELISA test kit can be used very effectively to test domestic sheep for infection with
A. ovis using the kit-supplied controls (i.e. the BcELISA) and a 19% inhibition cutoff; the kit may also be useful for detecting intra-erythrocytic
Anaplasma infections in wild ruminants.
Metrics
8 Record Views
Details
- Title
- Validation of an Anaplasma marginale cELISA for use in the diagnosis of A. ovis infections in domestic sheep and Anaplasma spp. in wild ungulates
- Creators
- Glen A Scoles - Animal Disease Research Unit, USDA-ARS, Pullman, WA 99164, USAWill L Goff - Animal Disease Research Unit, USDA-ARS, Pullman, WA 99164, USATimothy J Lysyk - Agriculture Agri-Foods Canada, Lethbridge Research Center, Lethbridge, Alberta, CanadaGregory S Lewis - United States Sheep Experiment Station, USDA-ARS, Dubois, ID, USADonald P Knowles - Animal Disease Research Unit, USDA-ARS, Pullman, WA 99164, USA
- Publication Details
- Veterinary microbiology, Vol.130(1), pp.184-190
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547762701842
- Language
- English
- Resource Type
- Journal article