Journal article
Variation in Biofilm Formation among Strains of Listeria monocytogenes
Applied and environmental microbiology, Vol.69(12), pp.7336-7342
12/2003
Handle:
https://hdl.handle.net/2376/107960
PMCID: PMC309931
PMID: 14660383
Abstract
Contamination of food by
Listeria monocytogenes
is thought to occur most frequently in food-processing environments where cells persist due to their ability to attach to stainless steel and other surfaces. Once attached these cells may produce multicellular biofilms that are resistant to disinfection and from which cells can become detached and contaminate food products. Because there is a correlation between virulence and serotype (and thus phylogenetic division) of
L. monocytogenes
, it is important to determine if there is a link between biofilm formation and disease incidence for
L. monocytogenes
. Eighty
L. monocytogenes
isolates were screened for biofilm formation to determine if there is a robust relationship between biofilm formation, phylogenic division, and persistence in the environment. Statistically significant differences were detected between phylogenetic divisions. Increased biofilm formation was observed in Division II strains (serotypes 1/2a and 1/2c), which are not normally associated with food-borne outbreaks. Differences in biofilm formation were also detected between persistent and nonpersistent strains isolated from bulk milk samples, with persistent strains showing increased biofilm formation relative to nonpersistent strains. There were no significant differences detected among serotypes. Exopolysaccharide production correlated with cell adherence for high-biofilm-producing strains. Scanning electron microscopy showed that a high-biofilm-forming strain produced a dense, three-dimensional structure, whereas a low-biofilm-forming strain produced a thin, patchy biofilm. These data are consistent with data on persistent strains forming biofilms but do not support a consistent relationship between enhanced biofilm formation and disease incidence.
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Details
- Title
- Variation in Biofilm Formation among Strains of Listeria monocytogenes
- Creators
- Monica K Borucki - Animal Disease Research Unit, Agricultural Research Service, United States Department of AgricultureJason D Peppin - Animal Disease Research Unit, Agricultural Research Service, United States Department of AgricultureDavid White - Animal Disease Research Unit, Agricultural Research Service, United States Department of AgricultureFrank Loge - Animal Disease Research Unit, Agricultural Research Service, United States Department of AgricultureDouglas R Call - Animal Disease Research Unit, Agricultural Research Service, United States Department of Agriculture
- Publication Details
- Applied and environmental microbiology, Vol.69(12), pp.7336-7342
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547263401842
- Language
- English
- Resource Type
- Journal article