Journal article
Visualization of endogenous hydrogen sulfide in living cells based on Au nanorods@silica enhanced fluorescence
Analytica chimica acta, Vol.1053, pp.81-88
04/11/2019
PMID: 30712572
Abstract
Hydrogen sulfide as a gas indicator molecule plays an important role in various human physiological processes. However, due to the high volatility and diffusivity of H2S in biological systems, it is very difficult to implement a precise assay for H2S detection. Compared with the destructive instrumental methods, assays based on fluorescence probes provide noninvasive and real-time detections of H2S in living cells. In this work, we presented a fluorescent nanoprobe based on dye-functionalized Au nanorods (NRs)@silica for sensitive and selective detection of H2S in vitro and living cells. With the metal enhanced fluorescence effect, the fluorescence turn-on and turn-off were controlled by the formation and disassembly of coordination compound between dyes and copper ions. Silica matrix was used to coat the Au NRs to prevent them from the biological cytotoxicity. The effects of the different distances between Au NRs and fluorophores on fluorescent enhancement were explored and approximately 5-fold fluorescence enhancement was obtained with a distance of 22 nm. A detection of limit of 17 nM was achieved. In addition, visualization of exogenous and endogenous H2S in living cells was validated.
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•A noninvasive fluorescence nanoprobe is constructed for the real-time detection of H2S in living cells.•Au@silica-TCPP nanoparticles take advantage of the metal enhanced fluorescence to achieve a high sensitivity.•Visualization of both the exogenous and endogenous H2S in living cells was validated.
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Details
- Title
- Visualization of endogenous hydrogen sulfide in living cells based on Au nanorods@silica enhanced fluorescence
- Creators
- Yanan Luo - Washington State UniversityYang Song - Washington State UniversityChengzhou Zhu - Washington State UniversitySuiqiong Li - Washington State UniversityMing Xian - Washington State UniversityChien M. Wai - University of IdahoYuehe Lin - Washington State UniversityDan Du - Washington State University
- Publication Details
- Analytica chimica acta, Vol.1053, pp.81-88
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Elsevier B.V
- Number of pages
- 8
- Identifiers
- 99901227640801842
- Language
- English
- Resource Type
- Journal article