Journal article
Vp1659 Is a Vibrio parahaemolyticus Type III Secretion System 1 Protein That Contributes to Translocation of Effector Proteins Needed To Induce Cytolysis, Autophagy, and Disruption of Actin Structure in HeLa Cells
Journal of bacteriology, Vol.192(13), pp.3491-3502
07/2010
Handle:
https://hdl.handle.net/2376/100875
PMCID: PMC2897656
PMID: 20418402
Abstract
Vibrio parahaemolyticus
harbors two type III secretion systems (T3SSs; T3SS1 and T3SS2), of which T3SS1 is involved in host cell cytotoxicity. T3SS1 expression is positively regulated by ExsA, and it is negatively regulated by ExsD. We compared the secretion profiles of a wild-type strain (NY-4) of
V. parahaemolyticus
with those of an ExsD deletion mutant (Δ
exsD
) and with a strain of NY-4 that overexpresses T3SS1 (NY-4:p
exsA
). From this comparison, we detected a previously uncharacterized protein, Vp1659, which shares some sequence homology with LcrV from
Yersinia
. We show that
vp1659
expression is positively regulated by ExsA and is negatively regulated by ExsD. Vp1659 is specifically secreted by T3SS1 of
V. parahaemolyticus
, and Vp1659 is not required for the successful extracellular secretion of another T3SS1 protein, Vp1656. Mechanical fractionation showed that Vp1659 is translocated into HeLa cells in a T3SS1-dependent manner and that deletion of Vp1659 does not prevent VopS from being translocated into HeLa cells during infection. Deletion of
vp1659
significantly reduces cytotoxicity when HeLa cells are infected by
V. parahaemolyticus
, while complementation of the Δ
vp1659
strain restores cytotoxicity. Differential staining showed that Vp1659 is required to induce membrane permeability in HeLa cells. We also show evidence that Vp1659 is required for actin rearrangement and the induction of autophagy. On the basis of these data, we conclude that Vp1659 is a T3SS1-associated protein that is a component of the secretion apparatus and that it is necessary for the efficient translocation of effector proteins into epithelial cells.
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Details
- Title
- Vp1659 Is a Vibrio parahaemolyticus Type III Secretion System 1 Protein That Contributes to Translocation of Effector Proteins Needed To Induce Cytolysis, Autophagy, and Disruption of Actin Structure in HeLa Cells
- Creators
- Xiaohui Zhou - Department of Veterinary Microbiology and PathologyMichael E Konkel - Department of Veterinary Microbiology and PathologyDouglas R Call - Department of Veterinary Microbiology and Pathology
- Publication Details
- Journal of bacteriology, Vol.192(13), pp.3491-3502
- Academic Unit
- Paul G. Allen School for Global Animal Health; Molecular Biosciences, School of
- Publisher
- American Society for Microbiology (ASM)
- Identifiers
- 99900546786101842
- Language
- English
- Resource Type
- Journal article