Wheat BAX inhibitor-1 contributes to wheat resistance to Puccinia striiformis

Xiaojie Wang; Chunlei Tang; Xueling Huang; Fangfang Li; Xianming Chen; Gang Zhang; Yanfei Sun; Dejun Han; Zhensheng Kang

doi:10.1093/jxb/ers140

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Wheat BAX inhibitor-1 contributes to wheat resistance to Puccinia striiformis

Journal article

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Wheat BAX inhibitor-1 contributes to wheat resistance to Puccinia striiformis

Xiaojie Wang, Chunlei Tang, Xueling Huang, Fangfang Li, Xianming Chen, Gang Zhang, Yanfei Sun, Dejun Han and Zhensheng Kang

Journal of experimental botany, Vol.63(12), pp.4571-4584

07/2012

DOI: https://doi.org/10.1093/jxb/ers140

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https://hdl.handle.net/2376/106652

PMID: 22696283

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Abstract

Triticum - immunology

Plant Components, Aerial - immunology

Plant Components, Aerial - physiology

Triticum - physiology

Basidiomycota - physiology

Plant Diseases - immunology

Molecular Sequence Data

Plant Roots - genetics

Seedlings - genetics

Plant Diseases - microbiology

Phylogeny

Tobacco - immunology

Plant Roots - physiology

Seedlings - immunology

Cell Death

Plant Proteins - metabolism

Basidiomycota - growth & development

Transgenes

Triticum - microbiology

Tobacco - physiology

Protein Structure, Tertiary

Amino Acid Sequence

Gene Expression

Disease Resistance

Gene Silencing

Gene Expression Regulation, Plant - genetics

Genotype

Plant Components, Aerial - genetics

Host-Pathogen Interactions

Triticum - genetics

Plant Proteins - genetics

Sequence Alignment

Seedlings - physiology

Tobacco - genetics

Plant Roots - immunology

BAX inihibitor-1 (BI-1) is proposed to be a cell death suppressor conserved in both animals and plants. The ability of BI-1 genes to inhibit programmed cell death (PCD) has been well studied in animals, but the physiological importance of BI-1 in plant-microbe interactions remains unclear. This study characterized BI-1 from wheat infected by Puccinia striiformis f. sp. tritici (Pst). The deduced TaBI-1 protein contained a Bax inhibitor domain and seven transmembrane regions conserved among members of the BI-1 family. Transcription of TaBI-1 was detected in all wheat tissues tested (culms, roots, leaves, anthers, and spikelets). Furthermore, TaBI-1 exhibited positive transcriptional responses to Pst infection and abiotic stresses. Overexpression of TaBI-1 in tobacco blocked Bax-induced cell death. Silencing TaBI-1 in plants of a resistant wheat genotype converted a resistant reaction to a relatively susceptible reaction when inoculated with an avirulent pathotype of the pathogen, and increased the area per infection site, but the percentage of necrotic cells did not change significantly, indicating that TaBI-1, a negative cell death regulator, contributes to wheat resistance to stripe rust. These results provide a better understanding of the molecular mechanism of wheat resistance to stripe rust.

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Title: Wheat BAX inhibitor-1 contributes to wheat resistance to Puccinia striiformis
Creators: Xiaojie Wang - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, PR China
Chunlei Tang
Xueling Huang
Fangfang Li
Xianming Chen
Gang Zhang
Yanfei Sun
Dejun Han
Zhensheng Kang
Publication Details: Journal of experimental botany, Vol.63(12), pp.4571-4584
Academic Unit: Plant Pathology, Department of
Publisher: England
Identifiers: 99900547058001842
Language: English
Resource Type: Journal article