Journal article
Wheat grain hardness results from highly conserved mutations in the friabilin components puroindoline a and b
Proceedings of the National Academy of Sciences - PNAS, Vol.95(11), pp.6262-6266
05/26/1998
Handle:
https://hdl.handle.net/2376/109178
PMCID: PMC27651
PMID: 9600953
Abstract
“Soft” and “hard” are the two main market classes of wheat (
Triticum aestivum
L.) and are distinguished by expression of the
Hardness
gene. Friabilin, a marker protein for grain softness (
Ha
), consists of two proteins, puroindoline a and b (pinA and pinB, respectively). We previously demonstrated that a glycine to serine mutation in pinB is linked inseparably to grain hardness. Here, we report that the pinB serine mutation is present in 9 of 13 additional randomly selected hard wheats and in none of 10 soft wheats. The four exceptional hard wheats not containing the serine mutation in pinB express no pinA, the remaining component of the marker protein friabilin. The absence of pinA protein was linked inseparably to grain hardness among 44 near-isogenic lines created between the soft variety Heron and the hard variety Falcon. Both pinA and pinB apparently are required for the expression of grain softness. The absence of pinA protein and transcript and a glycine-to-serine mutation in pinB are two highly conserved mutations associated with grain hardness, and these friabilin genes are the suggested tightly linked components of the
Hardness
gene. A previously described grain hardness related gene termed “
GSP-1
” (grain softness protein) is not controlled by chromosome 5D and is apparently not involved in grain hardness. The association of grain hardness with mutations in both pinA or pinB indicates that these two proteins alone may function together to effect grain softness. Elucidation of the molecular basis for grain hardness opens the way to understanding and eventually manipulating this wheat endosperm property.
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Details
- Title
- Wheat grain hardness results from highly conserved mutations in the friabilin components puroindoline a and b
- Creators
- Michael J Giroux - Plant, Soil and Environmental Sciences Department, P.O. Box 173120, Montana State University, Bozeman, MT 59717-312 (contribution no. J-5188 of the Montana Agricultural Experiment Station); andCraig F Morris - Plant, Soil and Environmental Sciences Department, P.O. Box 173120, Montana State University, Bozeman, MT 59717-312 (contribution no. J-5188 of the Montana Agricultural Experiment Station); and
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.95(11), pp.6262-6266
- Academic Unit
- Crop and Soil Sciences, Department of
- Publisher
- National Academy of Sciences
- Identifiers
- 99900547230901842
- Language
- English
- Resource Type
- Journal article