Journal article
Wheat transcription factor TaWRKY70 is positively involved in high‐temperature seedling plant resistance to Puccinia striiformis f. sp. tritici
Molecular plant pathology, Vol.18(5), pp.649-661
06/2017
Handle:
https://hdl.handle.net/2376/109816
PMCID: PMC6638234
PMID: 27145738
Abstract
Stripe rust, caused by Puccinia striiformis f. sp. tritici ( Pst ), is a devastating disease of wheat ( Triticum aestivum ) worldwide. Wheat high‐temperature seedling plant (HTSP) resistance to Pst is non‐race‐specific and durable. WRKY transcription factors have been proven to play important roles in plant defence responses to attacks by several pathogens. However, there is no direct evidence as to whether WRKY transcription factors play a role in HTSP resistance to Pst . We isolated a WRKY gene, named TaWRKY70 , from wheat cultivar Xiaoyan 6. The expression level of TaWRKY70 was increased significantly when exposed to high temperatures (HTs) during the initial symptom expression stage of Pst infection. The expression of this gene increased in plants treated with ethylene (ET), salicylic acid (SA) and cold (4°C) stresses, but decreased in plants treated with methyl jasmonate (MeJA) and heat (40°C) stresses. Silencing of TaWRKY70 led to greater susceptibility to Pst (in terms of the increase in length of uredinial pustules and the decrease in the number of necrotic cells) compared with non‐silenced plants when exposed to HT during the initial symptom expression stage of Pst infection, coinciding with expression changes of the ET‐ and SA‐responsive genes TaPIE1 and TaPR1 . 1 . In contrast, the expression level of the jasmonic acid (JA)‐responsive gene TaAOS was not affected by TaWRKY70 . These results indicate that TaWRKY70 is positively involved in HTSP resistance, during which SA and ET signalling are probably activated.
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Details
- Title
- Wheat transcription factor TaWRKY70 is positively involved in high‐temperature seedling plant resistance to Puccinia striiformis f. sp. tritici
- Creators
- Junjuan Wang - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F UniversityFei Tao - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F UniversityFei An - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F UniversityYiping Zou - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F UniversityWei Tian - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F UniversityXianming Chen - Washington State UniversityXiangming Xu - East Malling ResearchXiaoping Hu - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University
- Publication Details
- Molecular plant pathology, Vol.18(5), pp.649-661
- Academic Unit
- Department of Plant Pathology
- Publisher
- John Wiley and Sons Inc; Hoboken
- Number of pages
- 13
- Grant note
- 2013CB127700 / National Basic Research Program of China 31271985 / National Natural Science Foundation of China
- Identifiers
- 99900547222201842
- Language
- English
- Resource Type
- Journal article