Journal article
Whole Transcriptome Sequencing Analysis of the Synergistic Antimicrobial Effect of Metal Oxide Nanoparticles and Ajoene on Campylobacter jejuni
Frontiers in microbiology, Vol.9, pp.2074-2074
08/31/2018
Handle:
https://hdl.handle.net/2376/112805
PMID: 30233546
Abstract
Two metal oxide (i.e., Al
2
O
3
and TiO
2
) nanoparticles and ajoene, a garlic-derived organosulfur compound, were identified to be effective antimicrobials against
Campylobacter jejuni
, a leading cause of human gastrointestinal diseases worldwide. A significant synergistic antimicrobial effect was observed using ajoene and Al
2
O
3
/TiO
2
nanoparticles in a combined manner to cause at least 8 log
10
CFU/mL reduction of
C. jejuni
cells. Whole transcriptome sequencing (RNA-seq) and confocal micro-Raman spectroscopic analyses revealed the antimicrobial mechanism and identified the roles of ajoene and metal oxide nanoparticles in the synergistic treatment. Ajoene and metal oxide nanoparticles mediated a two-phase antimicrobial mechanism. Ajoene served as the inducing factor at the first phase that caused injury of cell membranes and increased the susceptibility of
C. jejuni
to stress. Metal oxide nanoparticles served as the active factor at the second phase that targeted sensitive cells and physically disrupted cell structure. This synergistic antimicrobial treatment demonstrates a potential to reduce the prevalence of
C. jejuni
and other pathogens on food contact surfaces and in the food chain.
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Details
- Title
- Whole Transcriptome Sequencing Analysis of the Synergistic Antimicrobial Effect of Metal Oxide Nanoparticles and Ajoene on Campylobacter jejuni
- Creators
- Rui Xue - Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai UniversityJinsong Feng - Food, Nutrition, and Health Program, Faculty of Land and Food Systems, The University of British ColumbiaLina Ma - Food, Nutrition, and Health Program, Faculty of Land and Food Systems, The University of British ColumbiaChunrong Liu - Department of Chemistry, Washington State UniversityMing Xian - Department of Chemistry, Washington State UniversityMichael E Konkel - School of Molecular Biosciences, College of Veterinary Medicine, Washington State UniversityShuo Wang - Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai UniversityXiaonan Lu - Food, Nutrition, and Health Program, Faculty of Land and Food Systems, The University of British Columbia
- Publication Details
- Frontiers in microbiology, Vol.9, pp.2074-2074
- Academic Unit
- Chemistry, Department of; Molecular Biosciences, School of
- Publisher
- Frontiers Media S.A
- Identifiers
- 99900547742701842
- Language
- English
- Resource Type
- Journal article