Journal article
Wild-type equine infectious anemia virus replicates in vivo predominantly in tissue macrophages, not in peripheral blood monocytes
Journal of virology, Vol.66(10), pp.5906-5913
10/1992
Handle:
https://hdl.handle.net/2376/113185
PMCID: PMC241467
PMID: 1382143
Abstract
In situ hybridization of tissues from two horses infected with the wild-type Wyoming strain of equine infectious anemia virus (EIAV) identified the liver, spleen, lymph nodes, kidney, lung, and adrenal gland as the primary host tissue sites for viral transcription during acute infection. Combined immunohistochemistry, with a monoclonal antibody recognizing a cytoplasmic antigen of equine mononuclear phagocytes, and in situ hybridization for viral RNA identified most infected cells as mature tissue macrophages. In contrast, in situ hybridization of adherent peripheral blood mononuclear cells collected from horses on various days during the first 2 weeks postinfection with the Wyoming strain of EIAV failed to detect any viral RNA in these cells. For the two horses described here, serum reverse transcriptase activity correlated directly with the degree of replication detected in tissue macrophages on the day of sacrifice. These results suggest that unlike other lentivirus infections in which mature tissue macrophages accumulate cytoplasmic viral RNA to a high level but fail to produce infectious virions, mature tissue macrophages are the likely primary source of the high titer of viremia present during acute infection with EIAV. No significant posttranscriptional block of viral replication in tissue macrophages appears to occur with EIAV.
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Details
- Title
- Wild-type equine infectious anemia virus replicates in vivo predominantly in tissue macrophages, not in peripheral blood monocytes
- Creators
- D C Sellon - Department of Microbiology, Pathology, North Carolina State University College of Veterinary Medicine, Raleigh 27606S T Perry - Department of Microbiology, Pathology, North Carolina State University College of Veterinary Medicine, Raleigh 27606L Coggins - Department of Microbiology, Pathology, North Carolina State University College of Veterinary Medicine, Raleigh 27606F J Fuller - Department of Microbiology, Pathology, North Carolina State University College of Veterinary Medicine, Raleigh 27606
- Publication Details
- Journal of virology, Vol.66(10), pp.5906-5913
- Academic Unit
- Veterinary Clinical Sciences, Department of
- Identifiers
- 99900547789401842
- Language
- English
- Resource Type
- Journal article