Journal article
Winged bean chymotrypsin inhibitors retard growth of Helicoverpa armigera
Gene, Vol.431(1), pp.80-85
2009
Handle:
https://hdl.handle.net/2376/104766
PMID: 19059315
Abstract
Two putative Kunitz-type chymotrypsin inhibitor genes (
WCI2 and
WCI5) were isolated from winged bean (
Psophocarpus tetragonolobus (L.) DC). While
WCI2 has previously been characterized,
WCI5 represents a new member of the WCI family.
WCI5 was exclusively expressed in winged bean seeds. Theoretical translation of both the genes resulted into polypeptides of 207 amino acids with 86% sequence similarity. The polypeptide sequences contain four half-cysteine residues and a well-conserved Leu
65–Ser
66 reactive site, typical for chymotrypsin inhibitors.
WCI5 and
WCI2 were expressed in
Pichia pastoris and the recombinant proteins were assayed against various proteinases. Both the inhibitors strongly inhibited commercially available bovine chymotrypsin. More importantly, gut proteinases of
Helicoverpa armigera larvae that damage many important crop plants, were inhibited by WCI2 and WCI5. In addition, both proteinase inhibitors demonstrated significant reduction of growth of
H. armigera larvae after feeding on inhibitor incorporated artificial diets. The inhibitory effects of WCI2 and WCI5 on activity of proteinases and larval growth make these proteins and their genes promising candidates for enhancing plant defense against
H. armigera using transgenic plants.
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Details
- Title
- Winged bean chymotrypsin inhibitors retard growth of Helicoverpa armigera
- Creators
- Manasi A Telang - School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, USAAshok P Giri - School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, USAPrashant S Pyati - Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411 008, IndiaVidya S Gupta - Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411 008, IndiaMechthild Tegeder - School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, USAVincent R Franceschi - School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, USA
- Publication Details
- Gene, Vol.431(1), pp.80-85
- Academic Unit
- Biological Sciences, School of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546765601842
- Language
- English
- Resource Type
- Journal article