Journal article
Zebrafish ( Danio rerio) vary by strain and sex in their behavioral and transcriptional responses to selenium supplementation
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, Vol.157(4), pp.310-318
2010
Handle:
https://hdl.handle.net/2376/104688
PMCID: PMC3047475
PMID: 20659579
Abstract
We used the Nadia, Gaighatta, Scientific Hatcheries, and TM1 zebrafish (
Danio rerio) strains to test the hypothesis that variation among populations influences the behavioral and transcriptional responses to selenium supplementation. When fed a diet with control levels of selenium, zebrafish strains differed significantly in behavior, characterized as their mean horizontal and vertical swimming positions within the tank. The four strains also differed in brain expression of selenoprotein P1a (
sepp1a), glutathione peroxidase 3 (
gpx3), thioredoxin reductase 1 (
txnrd1), and tRNA selenocysteine associated protein 1 (
secp43). Iodothyronine deiodinase 2 (
dio2) did not differ among strains but showed a sex-specific expression pattern. When supplemented with selenium, all strains spent a greater proportion of time near the front of the tank, but the response of vertical swimming depth varied by strain. Selenium supplementation also caused changes in selenoprotein expression in the brain that varied by strain for
sepp1a,
secp43, and
dio2, and varied by strain and sex for
txnrd1. Expression of
gpx3 was unaffected by selenium. Our data indicate that selenium homeostasis in the brain may be a regulator of behavior in zebrafish, and the strain-specific effects of selenium supplementation suggest that genetic heterogeneity among populations can influence the results of selenium supplementation studies.
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Details
- Title
- Zebrafish ( Danio rerio) vary by strain and sex in their behavioral and transcriptional responses to selenium supplementation
- Creators
- Maia J Benner - Department of Biological Sciences, University of Idaho, Moscow, ID, 83844-3051, USARobert E Drew - Department of Biological Sciences, University of Idaho, Moscow, ID, 83844-3051, USARonald W Hardy - Aquaculture Research Institute, Hagerman Fish Culture Experiment Station, University of Idaho, 3059 National Fish Hatchery Road # F, Hagerman, ID 83332-5851, USABarrie D Robison - Department of Biological Sciences, University of Idaho, Initiative for Bioinformatics in Evolutionary Studies, University of Idaho, Moscow, ID, 83844-3051, USA
- Publication Details
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, Vol.157(4), pp.310-318
- Academic Unit
- UNKNOWN
- Publisher
- Elsevier Inc
- Identifiers
- 99900546727901842
- Language
- English
- Resource Type
- Journal article