Zn- and Mg-doped hydroxyapatite nanoparticles for controlled release of protein

Sudip Dasgupta; Shashwat S Banerjee; Amit Bandyopadhyay; Susmita Bose

doi:10.1021/la903617e

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Zn- and Mg-doped hydroxyapatite nanoparticles for controlled release of protein

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Zn- and Mg-doped hydroxyapatite nanoparticles for controlled release of protein

Sudip Dasgupta, Shashwat S Banerjee, Amit Bandyopadhyay and Susmita Bose

Langmuir, Vol.26(7), pp.4958-4964

04/06/2010

DOI: https://doi.org/10.1021/la903617e

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https://hdl.handle.net/2376/109653

PMCID: PMC2862579

PMID: 20131882

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Abstract

Microscopy, Electron, Transmission

Magnesium - chemistry

Animals

Nanoparticles - chemistry

Nanoparticles - ultrastructure

Durapatite - chemistry

Serum Albumin, Bovine - chemistry

Spectroscopy, Fourier Transform Infrared

Zinc - chemistry

Bovine serum albumin (BSA) protein incorporated with hydroxyapatite (HA) nanoparticles (NPs) were synthesized by an in situ precipitation process. 2 mol % Zn(2+) and Mg(2+) were used as dopants to synthesize Zn(2+)/Mg(2+)-doped HA-BSA NPs. In our study we used BSA as a model protein. The amount of BSA uptake by doped and undoped HA NPs and subsequent release of BSA from NPs were investigated. Zn-doped HA NPs showed the highest amount of BSA uptake, whereas the amount of BSA loaded in undoped HA NPs was the lowest. A two-stage BSA release profile from doped and undoped HA NPs was observed in phosphate buffer solution (PBS) at pH 7.2 +/- 0.2. The initial burst release was due to the desorption of BSA from the HA surface. The later stage of slow release was controlled by the dissolution of BSA incorporated HA NPs. The BSA release rate from Zn-doped HA NPs was found to be the highest, whereas undoped HA NPs released BSA at the slowest rate. Our study showed that the protein release rate from HA NPs can be controlled by the addition of suitable dopants, and doped HA-based NP systems can be used in bone growth factor and drug release study.

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Title: Zn- and Mg-doped hydroxyapatite nanoparticles for controlled release of protein
Creators: Sudip Dasgupta - W M Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164-2920, USA
Shashwat S Banerjee
Amit Bandyopadhyay
Susmita Bose
Publication Details: Langmuir, Vol.26(7), pp.4958-4964
Academic Unit: Mechanical and Materials Engineering, School of
Publisher: United States
Grant note: R01 EB007351-01A1 / NIBIB NIH HHS R01 EB007351 / NIBIB NIH HHS R01-EB-007351 / NIBIB NIH HHS
Identifiers: 99900547002401842
Language: English
Resource Type: Journal article