Journal article
blaCMY-2-Positive IncA/C Plasmids from Escherichia coli and Salmonella enterica Are a Distinct Component of a Larger Lineage of Plasmids
Antimicrobial agents and chemotherapy, Vol.54(2), pp.590-596
02/2010
Handle:
https://hdl.handle.net/2376/106184
PMCID: PMC2812137
PMID: 19949054
Abstract
Large multidrug resistance plasmids of the A/C incompatibility complex (IncA/C) have been found in a diverse group of Gram-negative commensal and pathogenic bacteria. We present three completed sequences from IncA/C plasmids that originated from
Escherichia coli
(cattle) and
Salmonella enterica
serovar Newport (human) and that carry the cephamycinase gene
bla
CMY-2
. These large plasmids (148 to 166 kbp) share extensive sequence identity and synteny. The most divergent plasmid, peH4H, has lost several conjugation-related genes and has gained a kanamycin resistance region. Two of the plasmids (pAM04528 and peH4H) harbor two copies of
bla
CMY-2
, while the third plasmid (pAR060302) harbors a single copy of the gene. The majority of single-nucleotide polymorphisms comprise nonsynonymous mutations in
floR
. A comparative analysis of these plasmids with five other published IncA/C plasmids showed that the
bla
CMY-2
plasmids from
E. coli
and
S. enterica
are genetically distinct from those originating from
Yersinia pestis
and
Photobacterium damselae
and distal to one originating from
Yersinia ruckeri
. While the overall similarity of these plasmids supports the likelihood of recent movements among
E. coli
and
S. enterica
hosts, their greater divergence from
Y. pestis
or
Y. ruckeri
suggests less recent plasmid transfer among these pathogen groups.
Metrics
14 Record Views
Details
- Title
- blaCMY-2-Positive IncA/C Plasmids from Escherichia coli and Salmonella enterica Are a Distinct Component of a Larger Lineage of Plasmids
- Creators
- Douglas R Call - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WashingtonRandall S Singer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WashingtonDa Meng - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WashingtonShira L Broschat - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WashingtonLisa H Orfe - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WashingtonJanet M Anderson - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WashingtonDavid R Herndon - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WashingtonLowell S Kappmeyer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WashingtonJoshua B Daniels - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WashingtonThomas E Besser - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington
- Publication Details
- Antimicrobial agents and chemotherapy, Vol.54(2), pp.590-596
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health; Electrical Engineering and Computer Science, School of
- Publisher
- American Society for Microbiology (ASM)
- Identifiers
- 99900546872301842
- Language
- English
- Resource Type
- Journal article