d-Lactate dehydrogenase as a marker gene allows positive selection of transgenic plants

Judith Wienstroer; Martin K.M Engqvist; Hans-Henning Kunz; Ulf-Ingo Flügge; Veronica G Maurino

doi:10.1016/j.febslet.2011.11.020

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d-Lactate dehydrogenase as a marker gene allows positive selection of transgenic plants

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d-Lactate dehydrogenase as a marker gene allows positive selection of transgenic plants

Judith Wienstroer, Martin K.M Engqvist, Hans-Henning Kunz, Ulf-Ingo Flügge and Veronica G Maurino

FEBS letters, Vol.586(1), pp.36-40

2012

DOI: https://doi.org/10.1016/j.febslet.2011.11.020

Handle:

https://hdl.handle.net/2376/103411

PMID: 22155004

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Abstract

Arabidopsis thaliana

d-Lactate dehydrogenase

Plant transformation

Selection marker

► A novel selection marker system based on the metabolism of d-lactate was developed. ► At high d-lactate concentrations wild-type development is arrested after germination. ► Plants overexpressing d-lactate dehydrogenase detoxify d-lactate and survive. ► d-LDH was established as marker in Arabidopsis thaliana allowing selection after germination. ► Selection on d-lactate adds a marker system as a good alternative to traditional ones. d-Lactate negatively affects Arabidopsis thaliana seedling development in a concentration-dependent manner. At media d-lactate concentrations greater than 5–10 mM the development of wild-type plants is arrested shortly after germination whereas plants overexpressing the endogenous d-lactate dehydrogenase ( d-LDH) detoxify d-lactate to pyruvate and survive. When the transgenic plants are further transferred to normal growth conditions they develop indistinguishably from the wild type. Thus, d-LDH was successfully established as a new marker in A. thaliana allowing selecting transgenic plants shortly after germination. The selection on d-lactate containing media adds a new optional marker system, which is especially useful if the simultaneous selection of multiple constructs is desired.

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Title: d-Lactate dehydrogenase as a marker gene allows positive selection of transgenic plants
Creators: Judith Wienstroer - Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Universitätsstraße 1, 40225 Düsseldorf, Germany
Martin K.M Engqvist - Botanisches Institut, Biozentrum Köln, Universität zu Köln, Zülpicher Str. 47b, 50674 Cologne, Germany
Hans-Henning Kunz - Botanisches Institut, Biozentrum Köln, Universität zu Köln, Zülpicher Str. 47b, 50674 Cologne, Germany
Ulf-Ingo Flügge - Botanisches Institut, Biozentrum Köln, Universität zu Köln, Zülpicher Str. 47b, 50674 Cologne, Germany
Veronica G Maurino - Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Universitätsstraße 1, 40225 Düsseldorf, Germany
Publication Details: FEBS letters, Vol.586(1), pp.36-40
Academic Unit: Biological Sciences, School of
Publisher: Elsevier B.V
Identifiers: 99900546508801842
Language: English
Resource Type: Journal article