Journal article
oca2 regulation of chromatophore differentiation and number is cell type specific in zebrafish
Pigment cell and melanoma research, Vol.27(2), pp.178-189
03/2014
Handle:
https://hdl.handle.net/2376/106275
PMID: 24330346
Abstract
Summary
We characterized a zebrafish mutant that displays defects in melanin synthesis and in the differentiation of melanophores and iridophores of the skin and retinal pigment epithelium. Positional cloning and candidate gene sequencing link this mutation to a 410‐kb region on chromosome 6, containing the oculocutaneous albinism 2 (oca2) gene. Quantification of oca2 mutant melanophores shows a reduction in the number of differentiated melanophores compared with wildtype siblings. Consistent with the analysis of mouse Oca2‐deficient melanocytes, zebrafish mutant melanophores have immature melanosomes which are partially rescued following treatment with vacuolar‐type ATPase inhibitor/cytoplasmic pH modifier, bafilomycin A1. Melanophore‐specific gene expression is detected at the correct time and in anticipated locations. While oca2 zebrafish display unpigmented gaps on the head region of mutants 3 days post‐fertilization, melanoblast quantification indicates that oca2 mutants have the correct number of melanoblasts, suggesting a differentiation defect explains the reduced melanophore number. Unlike melanophores, which are reduced in number in oca2 mutants, differentiated iridophores are present at significantly higher numbers. These data suggest distinct mechanisms for oca2 in establishing differentiated chromatophore number in developing zebrafish.
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Details
- Title
- oca2 regulation of chromatophore differentiation and number is cell type specific in zebrafish
- Creators
- Alisha J Beirl - Washington State University VancouverTor H Linbo - University of WashingtonMarea J Cobb - Washington State University VancouverCynthia D Cooper - Washington State University Vancouver
- Publication Details
- Pigment cell and melanoma research, Vol.27(2), pp.178-189
- Academic Unit
- Molecular Biosciences, School of
- Number of pages
- 12
- Grant note
- Washington State University Vancouver American Cancer Society Institutional Research (IRG‐77‐003‐32) Melanoma Research Foundation
- Identifiers
- 99900547265101842
- Language
- English
- Resource Type
- Journal article