pH-responsive allochroic nanoparticles for the multicolor detection of breast cancer biomarkers

Weiqing Xu; Lei Jiao; Huarong Ye; Zhenzhong Guo; Yu Wu; Hongye Yan; Wenling Gu; Dan Du; Yuehe Lin; Chengzhou Zhu

doi:10.1016/j.bios.2019.111780

pH-responsive allochroic nanoparticles for the multicolor detection of breast cancer biomarkers

Weiqing Xu, Lei Jiao, Huarong Ye, Zhenzhong Guo, Yu Wu, Hongye Yan, Wenling Gu, Dan Du, Yuehe Lin Chengzhou Zhu

Biosensors & bioelectronics, Vol.148, p.111780

01/15/2020

: https://doi.org/10.1016/j.bios.2019.111780

: 31665670

Biotechnology & Applied Microbiology

Chemistry

Chemistry, Analytical

Life Sciences & Biomedicine

Nanoscience & Nanotechnology

Science & Technology

Biophysics

Electrochemistry

Physical Sciences

Estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor-2 (HER2) are the three crucial biomarkers for the clinical diagnosis of breast cancer. Sensitive and precise detection of ER, PR, and HER2 is of great significance for the diagnosis of breast cancer. Herein, through a simple solvent-induced self-assembly process, the self-carried allochroic nanoparticles were prepared by using some hydrophobic pH indicator molecules for allochroic NPs-linked immunosorbent assay (named ALISA) of ER, PR, and HER2, respectively. Meanwhile, the introduction of bovine serum albumin (BSA) and antibody (Ab) enhanced the dispersity of the self-assembled nanoparticles as signal tags. Since the ultra-high loading and high-efficiency release of pH indicators, the ALISA exhibitssatisfactory selectivity and sensitivity, which demonstrated the great potential in the early diagnosis and postoperative monitoring of breast cancers. Furthermore, the smartphone was introduced to combine with the ALISA for point-of-care testing, indicating the high feasibility in practical applications.

: pH-responsive allochroic nanoparticles for the multicolor detection of breast cancer biomarkers
: Weiqing Xu - Central China Normal University
Lei Jiao - Central China Normal University
Huarong Ye - China Resources (China)
Zhenzhong Guo - Wuhan University of Science and Technology
Yu Wu - Central China Normal University
Hongye Yan - Central China Normal University
Wenling Gu - Central China Normal University
Dan Du - Washington State University
Yuehe Lin - Washington State University
Chengzhou Zhu - Central China Normal University
: Biosensors & bioelectronics, Vol.148, p.111780
: School of Mechanical and Materials Engineering
: Elsevier
: 9
: Recruitment Program of Global Youth Experts of China CCNU18JCXK07 / self-determined research funds of CCNU from the college's basic research and operation of MOE B17019 / Program of Introducing Talents of Discipline to Universities of China (111 program) CCNU19QN066 / Fundamental Research Funds for the Central Universities
: 99901227641401842
: English
: Journal article